Effect of Vitamin A Supplementation on Growth Performance, Lipid Deposition, Antioxidant Ability, and Immunity in Juvenile Chinese Mitten Crab Eriocheir sinensis Fed Diet with Fish Oil Totally Replaced by Palm Oil

This research evaluated the protective effect of vitamin A (VA) on adverse fish oil (FO) substitution with palm (PO) in an economical crab Eriocheir sinensis. Three diets FO, PO, and PO + 8000 IU / kg VA as main lipid sources were fed to crabs, respectively, for 8 weeks. Compared crabs FO diet, PO diet showed reduced hemolymph concentration, feed utilization efficiency, growth performance. Besides, elevated content hepatopancreas body triglyceride hepatopancreas, leading decreased antioxidant enzyme immune parameters activities from biochemical analysis, enzymatic determination, quantitative polymerase chain reaction. In contrast, compared only, supplementation improved performance fatty acids deposition hepatopancreas. addition, suppressed gene expression related synthesis (dgat1) positively affected catabolism (cpt1a, cpt1b, cpt2, caat). Furthermore, upregulated genes (CuZnSOD CAT) through downregulating upstream regulator Keap1. (Lzm proPO) proinflammatory (LITAF, ADAM17, IL-16) Toll2/MyD88/Relish signaling pathway. study shows necessity addition totally replaced by because it can relieve PO’s effects improve crabs.